rabbit cell signaling technology her3 d22c5  (Cell Signaling Technology Inc)

Journal: Nature chemical biology

Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth

doi: 10.1038/s41589-021-00813-7

Figure Lengend Snippet: a) Immunoblot analysis of whole cell lysates from T47D cells treated with indicated compounds for 16 hours. Data are representative of at least n=2 independent experiments. b) Immunoblot analysis of lysates from T47D xenografts over a period of 24 hours following in vivo administration of a single dose of the indicated compounds. Data are representative of at least n=2 independent experiments.

Article Snippet: The following primary antibodies were used at 1:1000 dilution: anti-4E-BP1(53H11) #9644, anti-p-4EBP1 (Thr37/Thr46) (236B4) #2855, anti-p-4E-BP1 (Ser65) (174A9) #9456, anti-p-4E-BP1 (Ser65) (D9G1Q) #13443, anti-p-4E-BP1 (Thr70) (D756I) #13396, anti-4EBP2 #2845, anti-p-S6 Ribosomal Protein (Ser240/244) #2215, anti-p-S6 Ribosomal Protein (Ser240/244) (D68F8) #5364, anti-p-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) #4858, anti-p-p70 S6 Kinase (Thr389) #9205, anti-p70 S6 Kinase (49D7) #2708, anti-p70 S6 Kinase #9202, anti-p70 S6 Kinase 2 #14130, anti-p-AKT (Ser473) (D9E) #4060, anti-p-AKT (Ser473) #9271, anti-p-AKT (Thr308) (244F9) #4056, anti-AKT (11E7) #4685, anti-AKT (C67E7) #4691, anti-p-HER3 (Tyr1197) (C56E4) #4561, anti-HER3 (D22C5) #12708, anti-eIF4G1 #2858, anti-AKT #4691, anti-p-AKT (Ser473) #9271, anti-mTOR #2972, anti-β-actin (8H10D10) #3700, anti-HATag (C29F4) #3724, anti-FKBP51 (FKBP5) (D5G2) #12210 all from Cell Signaling Technologies (Danvers, MA, USA); anti-rpS6 (C-8) #sc-74459 from Santa Cruz Biotechnologies (Dallas, Texas, USA); anti-eIF4E #610269 from BD Biosciences (San Jose, CA, USA); anti-Cyclin D1 (17H3L3) #701421 from Thermo Fisher Scientific (Waltham, MA, USA); anti-FKBP12 #ab2918 (Abcam, Cambridge, UK); anti-β-actin (Clone AC-15) #A1978 from Sigma (Saint Louis, Missouri, USA); anti-puromycin (Clone 3RH11) #EQ0001 from Kerafast (Boston, MA, USA).

Techniques: In Vitro, In Vivo, Western Blot